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PCR reactions were carried out using a Techne PCR machine (Scientific Laboratory Supplies, Nottingham, UK) in a reaction containing 5 units Taq polymerase (Promega, Madison, WI, USA), 10.0 m m Tris‐HCl with pH of 9, 50.0 m m KCl, 0.1% Triton X‐100, 1.5 m m MgCl 2, 0.2 m m deoxynucleotide triphosphate (dNTP), 4.0 µ m forward primer and 4.0

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